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S9396
Sigma
Sphingomyelinase from Bacillus cereus
buffered aqueous glycerol solution, ≥100 units/mg protein (Lowry)
| Synonym: | Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase |
| CAS Number: | 9031-54-3 |
| Enzyme Commission (EC) Number: | 3.1.4.12 ( BRENDA | IUBMB ) |
| MDL number: | MFCD00132362 |
Description
| Unit Definition | One unit will hydrolyze 1.0 μmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C. |
| Physical form | Solution in 50% glycerol containing 50 mM Tris-HCl, pH 7.5. |
| Biochem/physiol Actions | Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin. |
Properties
| form | buffered aqueous glycerol solution |
| foreign activity | Phospholipase C ≤1% |
| storage temp. | 2-8°C |
Safety
| WGK Germany | 1 |
References
| reference | Raines, M.A., et al., Sphingomyelinase and ceramide activate mitogen-activated protein kinase in myeloid HL-60 cells. J. Biol. Chem. 268, 14572-14575, (1993) Abstract |
| Slotte, J.P., et al., Rapid turnover of plasma membrane sphingomyelin and cholesterol in baby hamster kidney cells after exposure to sphingomyelinase. Biochim. Biophys. Acta 1030, 251-257, (1990) Abstract | |
| Jungner, M., et al., Interfacial regulation of bacterial sphingomyelinase activity. Biochim. Biophys. Acta 1344, 230-240, (1997) Abstract |
